潮田研のこれまでの研究業績を紹介します。日本語の著書はこちらから。
*Corresponding Author

発表論文

Zn2+-dependent functional switching of ERp18, an ER-resident thioredoxin-like protein
Chika Tsutsumi#, Kaiku Uegaki#, Riyuji Yamashita, Ryo Ushioda*, Kazuhiro Nagata*
Cell Reports 43(2): 113682 (2024)

J-domain proteins: From molecular mechanisms to diseases
J. Marszalek, Ryo Ushioda, H. Kampinga* et al.
Cell Stress and Chaperones Volume 29, Issue 1 p21-33 (2024)

Tolerable glycometabolic stress boosts cancer cell resilience through altered N-glycosylation and Notch signaling activation.
Shungo Iwamoto, Takashi Kobayashi, Hisatoshi Hanamatsu, Ikuko Yokota, Yukiko Teranishi, Akiho Iwamoto, Miyu Kitagawa, Sawako Ashida, Ayane Sakurai, Suguru Matsuo, Yuma Myokan, Aiyu Sugimoto, Ryo Ushioda, Kazuhiro Nagata, Noriko Gotoh, Kazuki Nakajima, Takashi Nishikaze, Jun-ichi Furukawa & Naoki Itano*
Cell Death & Disease volume 15, Article number: 53 (2024)

Mechanistic characterization of disulfide bond reduction of an ERAD substrate mediated by cooperation between ERdj5 and BiP
Xiaohan Cai, Shogo Ito, Kentaro Noi, Michio Inoue, Ryo Ushioda, Yukinari Kato, Kazuhiro Nagata, Kenji Inaba*
J Biol Chem. 299(11):105274 (2023)
 
The oxidative folding of nascent polypeptides provides electrons for reductive reactions in the ER.
Kaiku Uegaki, Yuji Tokunaga, Michio Inoue, Seiji Takashima, Kenji Inaba, Koh Takeuchi, Ryo Ushioda*, Kazuhiro Nagata*
Cell Reports 42(7):112742 (2023)

Redox states in the endoplasmic reticulum directly regulate the activity of calcium channel, inositol 1,4,5-trisphosphate receptors
Shohei Fujii, Ryo Ushioda* and Kazuhiro Nagata*
Proc Natl Acad Sci U S A. 120(22):e2216857120 (2023)
 
Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells 
Hiroki Tanemura, Kenji Masuda, Takeshi Okumura, Eri Takagi, Daisuke Kajihara, Hirofumi Kakihara, Koichi Nonaka, Ryo Ushioda*
Scientific Reports 24;12(1);7239 (2022)

Ca2+ imbalance caused by ERdj5 deletion affects mitochondrial fragmentation
Riyuji Yamashita, Shohei Fujii, Ryo Ushioda* & Kazuhiro Nagata*
Scientific Reports 2;11(1):20772 (2021)

Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay
Michio Inoue, Nanami Sakuta, Satoshi Watanabe, Yuxia Zhang, Kunihito Yoshikaie, Yoshiki Tanaka, Ryo Ushioda, Yukinari Kato, Junichi Takagi, Tomoya Tsukazaki, Kazuhiro Nagata, Kenji Inaba*
Cell Reports 27, 4, 1221-1230 (2019)

Ryo Ushioda and Kazuhiro Nagata
Redox-Mediate Regulatory Mechanisms of Endoplasmic Reticulum Homeostasis
Cold Spring Harbor Perspectives in Biology　1;11(5) (2019)

Sasikumar, P., AlOuda, K. S., Kaiser, W. J., Holbrook, L. M., Kriek, N.,Unsworth, A. J., Bye, A. P., Sage, T., Ushioda, R., Nagata, K., Farndale, R. W., and Gibbins, J. M. 
 The chaperone protein HSP47: a platelet collagen binding protein that contributes to thrombosis and hemostasis.
Journal of thrombosis and hemostasis: JTH 16, 946-959, (2018) 

Caba, C., Ali Khan, H., Auld, J., Ushioda, R., Araki, K., Nagata, K., and Mutus, B. (2018)
Conserved Residues Lys(57) and Lys(401) of Protein Disulfide Isomerase Maintain an Active Site Conformation for Optimal Activity: Implications for Post-Translational Regulation.
Frontiers in molecular biosciences 5, 18 (2018) 

Maegawa, K., Watanabe, S., Noi, K., Okumura, M., Amagai, Y., Inoue, M., Ushioda, R., Nagata, K., Ogura, T. and Inaba, K.*
The highly dynamic nature of ERdj5 is key to efficient elimination of aberrant protein oligomers through ER-associated degradation
Structure, 25:846-857 (2017)

Araki K*, Ushioda R, Kusano H, Tanaka R, Hatta T, Fukui K, Nagata K, Natsume T
A crosslinker-based identification of redox relay targets
Anal. Biochem. 1;520:22-26. (2017)

R. Ushioda, A. Miyamoto, M. Inoue, S. Watanabe, M. Okumura, K. Maegawa, K. Uegaki, S. Fujii, Y. Fukuda, M. Umitsu, J. Takagi, K. Inaba, K. Mikoshibad, and K. Nagata*
Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5
Proc Natl Acad Sci U S A. Oct 11;113(41): E6055-E6063(2016)

この論文は産経新聞（2016年10月3日付23面)、京都新聞（2016年10月1日付33面）、共同通信、日経プレスリリースで報道された。
京都産業大学プレスリリース、理化学研究所プレスリリース (京産大へリンク)、東北大学プレスリリース、JSTプレスリリース

D.J. Klionsky*, K.Nagata, R.Ushioda et al.
Guidelines for the use and interpretation of assays for monitoring autophagy
Autophagy, 12(1): 1-222 (2016)

Kawasaki K, Ushioda R*, Ito S, Ikeda K, Masago Y, Nagata K*
Deletion of the Collagen-specific Molecular Chaperone Hsp47 Causes Endoplasmic Reticulum Stress-mediated Apoptosis of Hepatic Stellate Cells.
J Biol Chem. 290. 3639-3646 (2015)

Avezov E, Konno T, Zyryanova A, Chen W, Laine R, Crespillo-Casado A, Melo E, Ushioda R, Nagata K, Kaminski CF, Harding HP, Ron D*.
Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum 
BMC Biol. 10;13(1):2(2015)
 
R. Ushioda, J. Hoseki, K. Nagata*.
Glycosylation-independent ERAD patway serves as a backup system under ER stress.
Mol Biol Cell. 24(20):3155-63 (2013)

R. Ushioda and K. Nagata*.
The endoplasmic reticulum-associated degradation and disulfide reductase ERdj5.
Methods Enzymol. 490:235-58 (2011)

M. Hagiwara, K. Maegawa, M. Suzuki, R. Ushioda, K. Araki, Y. Matsumoto, J. Hoseki, K. Nagata* and K. Inaba*
Structural basis of an ERAD pathway mediated by the ER-resident protein disulfide reductase ERdj5.
Mol Cell. 41(4):432-444(2011) 

J. Hoseki, R. Ushioda and K. Nagata*.
Mechanism and components of endoplasmic reticulum-associated degradation.
Journal of Biochemistry 147(1):19-25(2010)

R.Ushioda, J.Hoseki, K.araki, G.Jansen , D.Y.Thomas, & K.Nagata*
ERdj5 is required as a disulfide reductase for degradation of misfolded proteins in the ER.
Science 321 (5888):569-72(2008)

この論文はBio Med Centralが提供するFACULTY OF 1000 BIOLOGYのMust Readに選定され、雑誌ACS CHEMICAL BIOLOGYのSpotlight（ACS CHEMICAL BIOLOGY vol.3 no.9 522-523, 2008）で特集された。国内でも読売新聞（2008年7月25日付2面）、朝日新聞（2008年7月25日付29面）、京都新聞（2008年7月25日付27面）などで報道された。